Leica SP5C Manuals

Manuals and User Guides for Leica SP5C. We have 1 Leica SP5C manual available for free PDF download: Operation Manual

Leica SP5C Operation Manual

Leica SP5C Operation Manual (32 pages)

Spectral Confocal Laser Scanning Microscope

Brand: Leica | Category: Microscope | Size: 1.15 MB

Table of Contents

Table of Contents
2
1 Starting up the SP5

3
2 Operation of the DMI6000 Inverted Microscope Base

5
3 Rules for the Use of the Mercury Vapour Arc Lamp

8
4 Image Acquisition Via the las AF Software

10
- Switching the Lasers on
  10
- Setting the USB Control Panel
  11
- Defining the Beam Path Settings
  12
- Generating New Beam Path Settings
  14
- Excitation Laser Line Selection
  14
- Spectrum View Tool
  14
- Spectroscopic Separation of the Emitted Fluorescence Signal
  14
- Setting the Slits for the Spectral Separation of the Emitted Light
  15
- Adjusting the PMT Settings
  16
- Additional Transmitted Light Channel
  18
- Saving and Loading of Beam Path Settings
  19
- Scanning Mode
  20
- Simultaneous Channel Acquisition
  20
- Sequential Channel Acquisition
  20
- Setting the Acquisition Mode
  22
- Setting the Scanning Parameters
  22
- Image Resolution
  22
- Pinhole Size
  23
- Scan Speed
  23
- Zoom
  23
- Averaging
  24
- Accumulation
  24
- Scan Field
  24
- Acquisition of Z-Stacks
  24
- Using the ROI Tool
  26
- Optimising Settings Via the USB Control Panel
  27
5 Saving and Exporting Data Files

28
6 Post-Acquisition Data Handling and Analysis

29
7 Shut-Down Procedure

30
8 Trouble Shooting

31

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