tecan Infinite 200 PRO Manuals

Manuals and User Guides for tecan Infinite 200 PRO. We have 1 tecan Infinite 200 PRO manual available for free PDF download: Instructions For Use Manual

tecan Infinite 200 PRO Instructions For Use Manual

tecan Infinite 200 PRO Instructions For Use Manual (192 pages)

DNA-Expert reader

Brand: tecan | Category: Laboratory Equipment | Size: 4.25 MB

Table of Contents

Table of Contents
5
1 Safety

9
- Instrument Safety
  9
2 General Description

11
- Instrument
  11
- Intended Use
  11
- Multifunctionality
  12
- Performance
  13
- User Friendliness
  13
- Onboard Control Button
  13
- Rear View
  14
- Software
  15
- Injectors (Optional)
  15
- Injector Measurement Modes
  15
- Injector Module Diagram
  16
- Injector Pump Options
  16
- Storage Bottles and Bottle Holders
  17
- Injector Carrier
  18
- Measurement Techniques
  20
- Fluorescence
  20
- Absorbance
  22
- Luminescence
  23
- Alphascreen/Alphalisa
  23
- Optical System
  24
- Fluorescence Intensity System (Infinite M200 PRO)
  24
- Fluorescence Intensity System (Infinite F200 PRO)
  30
- Fluorescence Polarization System (Infinite F200 PRO)
  34
- Absorbance System (Infinite F200 PRO)
  35
- Absorbance System (Infinite M200 PRO)
  37
- Luminescence System
  39
- Cuvette Port (Infinite M200 PRO)
  42
- Alphascreen/Alphalisa System (Infinite F200 PRO Only)
  45
3 Installation

47
- Unpacking and Inspection
  47
- Unpacking Procedure
  48
- Removal of the Transport Locks
  49
- Transport and Storage
  50
- Transport
  50
- Storage
  50
- Power Requirements
  51
- Switching the Instrument on
  52
4 Gas Control Module (Enhanced)

53
- Safety
  53
- Gas Control Module Features
  54
- Gas Control Module Configurations
  54
- Top and Rear Views of the Gas Control Module
  55
- Main Menu of Modes
  57
- CO2 Mode
  57
- O2 Mode
  58
- DUAL Mode
  59
- Manual Mode
  60
- Settings Menu
  61
- Installing the Gas Control Module
  62
- Requirements
  62
- Installation Procedure
  63
- Operating the Gas Control Module
  67
- CO and N Gas Cylinders (Not Supplied Accessory)
  71
- Troubleshooting the Gas Control Module
  72
5 Operating the Instrument

75
- Introduction
  75
- General Operating Features
  76
- Instrument Start up
  76
- General Options
  76
- Defining Filter Slides (Infinite F200 PRO Only)
  78
- About Filters
  78
- Filter Slide and Filter Orientation
  78
- Installing a Custom Filter
  80
- Defining the Filters
  83
- Optimizing Fluorescence Measurements
  87
- Instrument Parameters
  87
- Z-Optimization (FI Top Measurements with the Infinite M200 PRO Only)88
  88
- FI Ratio Mode
  93
- Optimal Read
  94
- FP Measurements
  96
- Fluorescence Polarization
  96
- Measurement Blank Range
  96
- G-Factor Settings
  97
- Measurement with an Uncalibrated G-Factor
  97
- Measurement with a Simultaneous G-Factor Calibration
  98
- Measurement with a Calibrated G-Factor
  99
- Measurement with a Manual G-Factor
  100
- Calculation of Fluorescence Polarization Parameters
  101
- Optimizing Absorbance Measurements
  102
- Measurement Parameters
  102
- Absorbance Ratio Mode
  102
- Multiple Reads Per Well
  103
- MRW Type
  103
- MRW Size
  104
- MRW Border
  104
- Result Display in MS Excel
  106
- Miscellaneous Software Features of MRW
  106
- Optimizing Luminescence Measurements
  107
- Integration Time
  107
- Light Level Attenuation
  107
- Optimizing Alphascreen/Alphalisa Measurements
  108
- 5.10.1 Instrument Parameters
  108
- Measurements with Injectors
  110
- 5.11.1 Priming and Washing of the Infinite 200 PRO
  110
- 5.11.2 Washing
  114
- 5.11.3 before Starting a Measurement with Injectors
  118
- Injector Modes (I-Control)
  118
- Blanking Measurements
  123
- Cuvette Measurements
  124
- 5.13.1 Cuvette Strip
  124
- 5.13.2 Cuvette Movements
  124
- 5.13.3 I-Control Cuvette Examples
  125
- I-Control Examples
  129
- Finishing a Measurement Session
  133
- 5.15.1 Disconnecting the Instrument
  133
- 5.15.2 Instrument Shut down
  133
6 Instrument Features

135
- Introduction
  135
- Instrument Specifications
  136
- Fluorescence Intensity and Time Resolved Fluorescence (TRF)
  138
- Definition of the Detection Limit
  139
- Fluorescein (Fluorescence Intensity) Top
  139
- Fluorescein (Fluorescence Intensity) Bottom
  139
- Europium (Time Resolved Fluorescence)
  139
- Fluorescence Polarization (FP)
  140
- Absorbance
  141
- Glow Type Luminescence
  142
- ATP Glow Luminescence
  142
- Flash Type Luminescence
  143
- Dual Color Luminescence (E.g. BRET)
  144
- Alphascreen/Alphalisa
  144
- On the Fly" Measurements
  145
- Cuvette Features (Infinite M200 PRO Only)
  146
- 6.11.1 Cuvette Specifications
  146
- Injector Specifications
  147
- 6.12.1 Injector Reagent Compatibility
  147
- Measurement Accessories
  148
- Recommended Filters (Infinite F200 PRO Only)
  148
- 6.13.2 Recommended Types of Microplates
  149
- 6.13.3 Luminescence Detection
  153
7 Quality Control

155
8 Cleaning and Maintenance

177
- Introduction
  177
- Liquid Spills
  178
- Injector Cleaning and Maintenance
  178
- Daily Maintenance
  179
- Weekly/Periodical Maintenance
  179
- Instrument Disinfection
  180
- Disinfection Solutions
  180
- Disinfection Procedure
  181
- Safety Certificate
  181
- Disposal
  182
- Disposal of Packing Material
  182
- Disposal of Operating Material
  182
- Disposal of the Instrument
  183
9 Troubleshooting

185
Index

189

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